Mouse IL-10 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4172
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser19-Ser178
Accession # NP_034678
Specificity
Clonality
Host
Isotype
Applications for Mouse IL-10 Antibody
ELISA
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha-helical cytokines that also includes IL‑19, IL-20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%-77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL-10 R alpha/IL-10 R1 chains, resulting in recruitment of two IL-10 R beta/IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
References
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
- Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
- Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
- Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
- Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
- Hsu, D.-H. et al. (1990) Science 250:830.
- Moore, K. et al. (1990) Science 248:1230.
- Windsor, W.T. et al. (1993) Biochemistry 32:8807.
- Syto, R. et al. (1998) Biochemistry 37:16943.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
- Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
- Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
- Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
- Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional IL-10 Products
Product Documents for Mouse IL-10 Antibody
Product Specific Notices for Mouse IL-10 Antibody
For research use only