C57BL/6 mouse bone marrow cells differentiated into dendritic cells by treatment with 20 ng/mL Recombinant Mouse GM-CSF (Catalog # 415-ML) for 5 days, 1 µg/mL LPS on day 6 and harvested on day 7 were stained with Rat Anti-Mouse IL-15 Ra Monoclonal Antibody (Catalog # MAB5511, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse IL-15R alpha Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: C57BL/6 mouse bone marrow cells differentiated into dendritic cells by treatment with 20 ng/mL Recombinant Mouse GM‑CSF (Catalog # 415-ML) and LPS

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB5511 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL-15 R alpha) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non‑lymphoid cells. IL-15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta and IL-2 R gammac, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha‑mediated apoptosis in fibroblasts by competing with TNFRI for TRAF2 binding.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

Q60819

Additional IL-15R alpha Products

Product Documents for Mouse IL-15R alpha Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse IL-15R alpha Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IL-15R alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5511

Key Product Details

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Species Reactivity

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Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IL-15R alpha Antibody

Detection of IL‑15 R alpha in C57BL/6 Mouse Bone Marrow-derived Dendritic Cells by Flow Cytometry.