Tramp-C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL-17C Monoclonal Antibody (Catalog # MAB23061, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse IL-17C Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Tramp-C1 mouse prostate cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17C

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1‑4). The functions of IL-17 RC, D, and E are not known.

Mouse IL-17C cDNA encodes a 194 amino acid (aa) residues protein with a putative 14 aa signal peptide (5). Although there are no potential N-linked glycosylation sites, it is reportedly glycosylated (6). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Mouse and human IL-17C share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) in an adult prostate and fetal kidney libraries (2). IL-17C has been shown to stimulate the release of TNF-alpha and IL-1 beta from the monocytic cell line THP-1, a property it shares with IL-17B (6, 7). Human IL‑17C is active on mouse cells (5). The receptor of IL-17C has not yet been identified. The IL-17C preparations from R&D Systems have been found to bind immobilized recombinant mouse IL-17B R/Fc in a functional ELISA.

References

Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Hurst, S.D. et al. (2002) J. Immunology 169:443.
Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.

Long Name

Interleukin 17C

Alternate Names

Cytokine CX2, IL17C

Entrez Gene IDs

27189 (Human); 234836 (Mouse)

Gene Symbol

IL17C

UniProt

NP_665833

Additional IL-17C Products

Product Documents for Mouse IL-17C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-17C Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse IL-17C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB23061

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IL-17C Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IL-17C Antibody

Detection of IL‑17C in Tramp‑C1 Mouse Cell Line by Flow Cytometry.