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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 130122

Product Summary for Mouse IL-2 R beta Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-2 R beta
Ala27-Glu240
Accession # P16297

Specificity

Detects mouse IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rhIL-2 R beta, recombinant mouse (rm) IL-2 R gamma, rhIL-15 R alpha, or rmIL-21 R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL-2 R beta Antibody

Detection of IL-2 R beta antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑2 R beta in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with anti-mouse Integrin a2/CD49b APC-conjugated antibody and either (A) Rat Anti-Mouse IL-2 R beta Monoclonal Antibody (Catalog # MAB5891) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse IL-2 R beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

IL‑2 R beta (IL-2 Receptor beta; designated CD122) is a type I transmembrane protein that associates with IL-2 R alpha and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. IL‑2 R beta and the common gamma chain can also associate with IL-15 R alpha. IL-2 R beta is expressed by T, B, NK cells and monocyte/macrophages.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Gene Symbol

IL2RB

UniProt

Additional IL-2 R beta Products

Product Documents for Mouse IL-2 R beta Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-2 R beta Antibody

For research use only

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