Mouse IL-27 p28/IL-30 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1834
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
ELISA Capture, ELISA Development, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
Phe29-Ser234
Accession # Q8K3I6
Phe29-Ser234
Accession # Q8K3I6
Specificity
Detects mouse IL-27 p28/IL-30 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse IL-27 p28/IL-30 Antibody
IL‑27 p28/IL‑30 in Mouse Thymus.
IL-27 p28/IL-30 was detected in perfusion fixed paraffin-embedded sections of mouse thymus using 15 µg/mL Mouse IL-27 p28/ IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
IL‑27 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑27 p28/IL‑30 Antibody.
Recombinant Mouse IL-27 (Catalog # 2799-ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IL-27 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-27 p28/IL-30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1834). The ND50 is typically 1-5 µg/mL.Applications for Mouse IL-27 p28/IL-30 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of mouse thymus
Sample: Perfusion fixed paraffin-embedded sections of mouse thymus
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IL‑27 p28/IL-30
Sample: Recombinant Mouse IL‑27 p28/IL-30
Neutralization
Measured by its ability to neutralize IL‑27 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Bender, H. et al. (2009) Hepatology 50:585. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑27.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-27 p28/IL-30
Long Name
Interleukin 27 p28
Alternate Names
IL-30, IL27 p28
Gene Symbol
IL27
UniProt
Additional IL-27 p28/IL-30 Products
Product Documents for Mouse IL-27 p28/IL-30 Antibody
Product Specific Notices for Mouse IL-27 p28/IL-30 Antibody
For research use only
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