Mouse IL-27 p28/IL-30 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF1834

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Direct ELISA, ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-27 p28/IL-30 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-27 p28/IL-30
Phe29-Ser234
Accession # Q8K3I6

Specificity

Detects mouse IL-27 p28/IL-30 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse IL-27 p28/IL-30 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑27 p28/IL-30

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV‑induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2‑4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N‑linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL‑12), IL‑27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N‑linked glycosylation site and four alpha‑helices, placing it in the IL‑6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL‑27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL‑27 binds to and signals through a heterodimeric receptor complex composed of WSX‑1 (TCCR) and gp130. Evidence suggests IL‑27 interacts only with WSX‑1 (7, 9, 10). IL‑27 has both anti-  and proinflammatory properties. As an anti‑inflammatory, IL‑27 seems to induce a general negative feedback program that limits T  and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activit  (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL‑27 secretion through TLR4, and also block IL-27 production via C5a induction (11).

References

  1. Boulay, J-L. et al. (2003) Immunity 19:159.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Murakami, M. et al. (2004) Growth Factors 22:75.
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Expert Opin. Biol. Ther. 3:715.
  5. Nomura, H. et al. (1997) GenBank Accession # O35228.
  6. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl). 193:1.
  7. Pflanz, S. et al. (2002) Immunity 16:779.
  8. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  9. Pflanz, S. et al. (2004) J. Immunol. 172: 2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hawlish, H. et al. (2005) Immunity 22:415.

Long Name

Interleukin 27 p28

Alternate Names

IL-30, IL27 p28

Entrez Gene IDs

246778 (Human); 246779 (Mouse)

Gene Symbol

IL27

UniProt

Q8K3I6

Additional IL-27 p28/IL-30 Products

Product Documents for Mouse IL-27 p28/IL-30 Biotinylated Antibody

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Product Specific Notices for Mouse IL-27 p28/IL-30 Biotinylated Antibody

For research use only

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