Mouse Integrin beta4/CD104 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4054
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta4/CD104
Asn29-Cys707
Accession # NP_001005608
Asn29-Cys707
Accession # NP_001005608
Specificity
Detects mouse Integrin beta4/CD104 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Integin beta4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Integrin beta4/CD104 Antibody
Detection of Mouse Integrin beta4/CD104 by Western Blot.
Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Integrin beta4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4054) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Three specific bands were detected for Integrin beta4/CD104 at approximately 150-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse Integrin beta4/CD104 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse skin tissue
Sample: Mouse skin tissue
Reviewed Applications
Read 1 review rated 5 using AF4054 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Integrin beta4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha6. Mature mouse Integrin beta4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta4 shares 88% and 96% aa identity with human and rat beta4, respectively.
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
UniProt
Additional Integrin beta 4/CD104 Products
Product Documents for Mouse Integrin beta4/CD104 Antibody
Product Specific Notices for Mouse Integrin beta4/CD104 Antibody
For research use only
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