Mouse L1CAM Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5674

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry

Cited:

ELISA Capture, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 555

View all L1CAM Antibodies »

Product Specifications

Immunogen

Mouse cerebellum-derived partially purified L1CAM

Specificity

Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse L1CAM Antibody

Detection of L1CAM antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of L1CAM in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B).

Detection of L1CAM in Neuro-2A cells by Flow Cytometry.

Neuro-2A cells were stained with Rat Anti-Mouse L1CAM Monoclonal Antibody (Catalog # MAB5674, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse L1CAM Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes and Neuro-2A mouse neuroblastoma cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L1CAM

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). L1CAM is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM (2). Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail (3). Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins (4). L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub (4). The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis (1). Overexpression promotes tumor cell invasion and motility, growth in nude mice, and tumor metastasis (1). Proteolytic processing by ADAM10 and
presenilin/ gamma-secretase is essential for the nuclear signaling of L1CAM in human carcinoma cell lines (1). Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome (5, 6).

References

  1. Riedle, S. et al. (2009) Biochem. J. 420:391.
  2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
  3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
  4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
  5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
  6. Striha, L. et al. (2000) J. Child Neurol. 15:239.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human); 16728 (Mouse); 50687 (Rat)

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for Mouse L1CAM Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse L1CAM Antibody

For research use only

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