Mouse LAIR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10092

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB10092-100
MAB10092-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2459C

View all LAIR1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived mouse LAIR1
Gln22-Tyr141 & Ser25-Tyr141
Accession # Q8BG84

Specificity

Detects mouse LAIR1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse LAIR1 Antibody

Detection of Mouse LAIR1 antibody by Western Blot.

Detection of Mouse LAIR1 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and mouse thymus tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse LAIR1 Monoclonal Antibody (Catalog # MAB10092) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for LAIR1 at approximately 36-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse LAIR1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line and mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR1c lacks a signal sequence, and LAIR1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR1 shows high‑affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR1 ECD also shares 62%, 31% and 28% aa identity with rat, canine, and bovine orthologs, respectively.

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Q8BG84

Additional LAIR1 Products

Product Documents for Mouse LAIR1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse LAIR1 Antibody

For research use only

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