Mouse Leptin R Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB497
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Leu21-Leu840 (predicted)
Accession # P48356
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse Leptin R Antibody
Detection of Leptin R in HEK293 Human Cell Line transfected with Mouse Leptin R and EGFP by Flow Cytometry.
HEK293 human cell line transfected with Mouse Leptin R and EGFP was stained with (A) Rat Anti-Mouse Leptin R Biotinylated Monoclonal Antibody (Catalog # MAB497) or (B) isotype control antibody (MAB006), followed by Allophycocyanin-conjugated anti-Rat IgG Secondary Antibody (F0113). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Mouse Leptin R Antibody
Flow Cytometry
Sample: HEK293 human cell line transfected with mouse Leptin R and EGFP
Mouse Leptin R Sandwich Immunoassay
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Leptin R
Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R is encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype. Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R binds leptin with high affinity and is a potent leptin antagonist.
References
- Tartaglia, L.A. et al. (1995) Cell 83:1263.
- Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
- Lee, J. I. and J.M. Friedman (1996) Nature 379:632.
- Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
- Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Leptin R Products
Product Documents for Mouse Leptin R Antibody
Product Specific Notices for Mouse Leptin R Antibody
For research use only