Mouse LIFR alpha APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5990A

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 673602

View all LIFR alpha Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
Leu44-Ser828
Accession # P42703

Specificity

Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse LIFR alpha APC-conjugated Antibody

Detection of LIF Ra antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB5990A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse LIFR alpha APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: D3 mouse embryonic stem cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta (due to its participation in signal transduction) and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).

References

  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Tomida, M. et al. (1994) J. Biochem. 115:557.
  4. Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
  5. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci.  USA 89:8616.
  6. Gearing, D.P. et al. (1992) Science 255:1434.
  7. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  8. Ip, N.Y. et al. (1992) Cell 69:1121.
  9. Davis, S. et al. (1993) Science 260:1805.
  10. Metcalf, D (2003) Stem Cells 21:5.
  11. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  12. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  13. Stewart, C.L. et al. (1992) Nature 359:76.
  14. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

P42703

Additional LIFR alpha Products

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Product Specific Notices for Mouse LIFR alpha APC-conjugated Antibody

For research use only

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