D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB5990P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse LIFR alpha PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: D3 mouse embryonic stem cell line

Reviewed Applications

Read 1 review rated 5 using FAB5990P in the following applications:

Flow Cytometry (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta and CD118, is a 185-190 kDa type I transmembrane protein that belongs to the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M. Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain. Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD. LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity. Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130. The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M. gp130 and LIF R alpha also associate with different ligand-specific receptors to form signaling receptor complexes for other IL-6 family ligands such as CNTF, CT-1 and CLC. The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha. LIF R alpha is widely expressed, and cells reported to contain LIF R alpha include hepatic sinusoidal endothelium, steroidal adrenal cortical cells, uterine luminal columnar epithelium, cardiac muscle cells, embryonic stem cells, odontoblasts, forebrain neural precursors, fibroblasts (3T3), osteoblasts, megakaryocytes, osteocytes, activated macrophages and sympathetic neurons. LIF induces the proliferation, differentiation, and activation of cells in many tissues. In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

P42703

Additional LIFR alpha Products

Product Documents for Mouse LIFR alpha PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse LIFR alpha PE-conjugated Antibody

For research use only

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Flow Cytometry

Mouse LIFR alpha PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5990P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

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Cited:

Label

Antibody Source

View all LIFR alpha Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse LIFR alpha PE-conjugated Antibody

Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry.