Mouse Lipocalin-2/NGAL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1857
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 228418
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2
aa 21-200
Accession # P11672
aa 21-200
Accession # P11672
Specificity
Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Lipocalin-2/NGAL Antibody
Detection of Mouse Lipocalin‑2/NGAL by Western Blot.
Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin-2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin-2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Lipocalin-2/NGAL Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue
Sample: Mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lipocalin-2/NGAL
Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores.
Long Name
Neutrophil Gelatinase-associated Lipocalin
Alternate Names
24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin
Gene Symbol
LCN2
UniProt
Additional Lipocalin-2/NGAL Products
Product Documents for Mouse Lipocalin-2/NGAL Antibody
Product Specific Notices for Mouse Lipocalin-2/NGAL Antibody
For research use only
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