Mouse MGMT Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3299
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 300008
Product Specifications
Immunogen
E. coli-derived recombinant mouse MGMT
Met1-Asn211
Accession # P26187
Met1-Asn211
Accession # P26187
Specificity
Detects mouse MGMT in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse MGMT Antibody
Detection of Mouse MGMT by Western Blot.
Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse MGMT Monoclonal Antibody (Catalog # MAB3299) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MGMT at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse MGMT Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Balb/3T3 mouse embryonic fibroblast cell line
Sample: Balb/3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MGMT
The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O6 position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.
Long Name
O-6 Methylguanine-DNA Methyltransferase
Alternate Names
6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase
Gene Symbol
MGMT
UniProt
Additional MGMT Products
Product Documents for Mouse MGMT Antibody
Product Specific Notices for Mouse MGMT Antibody
For research use only
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