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Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody

R&D Systems, part of Bio-Techne | Catalog # AF753

R&D Systems, part of Bio-Techne
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AF753
AF753-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NRG3
Glu284-Pro344
Accession # O35181

Specificity

Detects mouse NRG3 EGF-like domain in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody

Neuregulin-3/NRG3 antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Neuregulin‑3/NRG3 in Mouse Brain.

Neuregulin-3/NRG3 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Mouse Neuregulin-3/NRG3 EGF-like Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF753) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Neuregulin-3/NRG3 EGF-like Domain

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuregulin-3/NRG3

The neuregulin (also known as heregulin) cytokine family is comprised of four genes that encode a large number of secreted or membrane-bound isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. The NRG1 gene is the most pleiotropic, encoding more than 14 soluble or transmembrane proteins. NRG1 isoforms can be classified into type I (neu differentiation factor, NDF; heregulin, HRG; acetylcholine receptor inducing activity, ARIA), type II (glial growth factor, GGF) and type III (sensory and motor neuron-derived factor, SMDF) isoforms. NRG proteins show distinct spatial and temporal expression patterns and play important roles during development of both the nervous system and the heart.

Alternate Names

Neuregulin3, NRG3

Entrez Gene IDs

10718 (Human); 18183 (Mouse)

Gene Symbol

NRG3

UniProt

Additional Neuregulin-3/NRG3 Products

Product Documents for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody

For research use only

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