Mouse Neutrophil Elastase/ELA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4517
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 887105
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neutrophil Elastase/ELA2
Met1-Arg260
Accession # Q3UP87
Met1-Arg260
Accession # Q3UP87
Specificity
Detects mouse Neutrophil Elastase/ELA2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Neutrophil Elastase/ELA2 Antibody
Detection of Mouse Neutrophil Elastase/ELA2 by Western Blot.
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB4517) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Neutrophil Elastase/ELA2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Neutrophil Elastase/ELA2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Mouse bone marrow
Sample: Mouse bone marrow
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB4517 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neutrophil Elastase/ELA2
Alternate Names
ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin
Gene Symbol
ELANE
UniProt
Additional Neutrophil Elastase/ELA2 Products
Product Documents for Mouse Neutrophil Elastase/ELA2 Antibody
Product Specific Notices for Mouse Neutrophil Elastase/ELA2 Antibody
For research use only
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