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Mouse Notch-2 Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5196T

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 605724

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2
Leu26-Val528 (predicted)
Accession # O35516

Specificity

Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity with rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinant rat (rr) Notch2, or rrDLL1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse Notch-2 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Notch-2

Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins.  It is found on multiple cell types and exhibits multiple functions.  On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation.  Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor.  There are three potential splice variants.  One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713.  Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.   

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human); 29492 (Rat)

Gene Symbol

NOTCH2

UniProt

Additional Notch-2 Products

Product Documents for Mouse Notch-2 Alexa Fluor® 594-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Notch-2 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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