Mouse Notch-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5196
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 605724
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2
Leu26-Val528 (predicted)
Accession # O35516
Leu26-Val528 (predicted)
Accession # O35516
Specificity
Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100%
cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity
with rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinant
rat (rr) Notch2, or rrDLL1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Notch-2 Antibody
Detection of Notch‑2 in C2C12 Mouse Cell Line by Flow Cytometry.
C2C12 mouse myoblast cell line was stained with Mouse Notch-2 Monoclonal Antibody (Catalog # MAB5196, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).Applications for Mouse Notch-2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: C2C12 mouse myoblast cell line
Sample: C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-2
Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins. It is found on multiple cell types and exhibits multiple functions. On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation. Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470). Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor. There are three potential splice variants. One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713. Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.
Additional Notch-2 Products
Product Documents for Mouse Notch-2 Antibody
Product Specific Notices for Mouse Notch-2 Antibody
For research use only
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