Mouse Podocalyxin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1556
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Bioassay, Co-Immunoprecipitation, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 192703
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Ser21-Arg402
Accession # Q9R0M4
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Podocalyxin Antibody
Podocalyxin in bEnd.3 Mouse Cell Line.
Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Podocalyxin in Mouse Kidney Tissue.
Podocalyxin was detected in perfusion fixed frozen sections of mouse kidney tissue using Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse Podocalyxin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: D3 mouse embryonic stem cell line
Sample: D3 mouse embryonic stem cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line
Immunohistochemistry
1-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney tissue
Sample: Perfusion fixed frozen sections of mouse kidney tissue
Western Blot
1 µg/mL
Sample: Recombinant Mouse Podocalyxin
Sample: Recombinant Mouse Podocalyxin
Reviewed Applications
Read 3 reviews rated 5 using MAB1556 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
References
- Li, J. et al. (2001) DNA Seq. 12(5):407.
- Hara, T. et al. (1999) Immunity 11(5):567.
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
UniProt
Additional Podocalyxin Products
Product Documents for Mouse Podocalyxin Antibody
Product Specific Notices for Mouse Podocalyxin Antibody
For research use only
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