bEnd.3 mouse brain endothelial cell line was stained with Rat Anti-Mouse Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1556P, filled histogram) or isotype control antibody (IC013P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Detection of Podocalyxin in Undifferentiated D3 Mouse Cell Line by Flow Cytometry.

Undifferentiated D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1556P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Podocalyxin PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Undifferentiated D3 mouse embryonic stem cell line and bEnd.3 mouse brain endothelial cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References

Li, J. et al. (2001) DNA Seq. 12(5):407.
Hara, T. et al. (1999) Immunity 11(5):567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

Q9R0M4

Additional Podocalyxin Products

Product Documents for Mouse Podocalyxin PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Podocalyxin PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Mouse Podocalyxin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1556P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Podocalyxin PE-conjugated Antibody

Detection of Podocalyxin in bEnd.3 Mouse Cell Line by Flow Cytometry.