Mouse Pro‑MMP‑7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB29671
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 377313
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP-7
Leu18-Leu264
Accession # AAA99983
Leu18-Leu264
Accession # AAA99983
Specificity
Detects the pro form of mouse MMP-7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse MMP‑2, -3, -8, -9, -12, or -24 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Pro‑MMP‑7 Antibody
Detection of Mouse Pro‑MMP‑7 by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line transfected with with mouse MMP-7. PVDF Membrane was probed with 2 µg/mL of Mouse Pro-MMP-7 Monoclonal Antibody (Catalog # MAB29671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, active rmMMP-7 was derived from the proteolytic activation of Recombinant Mouse MMP-7 (Catalog # 2967-MP) (20 ng/lane) and probed with 1 µg/mL of Mouse MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967). Specific bands were detected for Pro-MMP-7 at approximately 35 kDa and active MMP-7 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Pro‑MMP‑7 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: NS0 mouse myeloma cell line transfected with with mouse MMP-7
Sample: NS0 mouse myeloma cell line transfected with with mouse MMP-7
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-7
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP‑7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin, and versican (1). MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha‑defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
References
- Woessner, J.F. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 532.
Long Name
Matrix Metalloproteinase 7
Alternate Names
Matrilysin, MMP7
Gene Symbol
MMP7
UniProt
Additional MMP-7 Products
Product Documents for Mouse Pro‑MMP‑7 Antibody
Product Specific Notices for Mouse Pro‑MMP‑7 Antibody
For research use only
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