Mouse Protein S/PROS1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4976
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 818002
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Protein S/PROS1
Met1-Arg669
Accession # Q08761
Met1-Arg669
Accession # Q08761
Specificity
Detects mouse Protein S/PROS1 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human Protein S is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Protein S/PROS1 Antibody
Detection of Mouse Protein S/PROS1 by Western Blot.
Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4976) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein S/PROS1 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Protein S/PROS1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Sample: RAW 264.7 mouse monocyte/macrophage cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein S/PROS1
Protein S (PROS1) is a 70-75 kDa plasma glycoprotein member of the vitamin K dependent family of proteins. It is synthesized by hepatocytes, Sertoli cells, macrophages, vascular smooth muscle cells and endothelium. Protein S is a non-enzymatic cofactor for activated protein C and participates in the degradation of coagulation factors FVa and FVIIIa. It also binds to Axl on smooth muscle, likely initiating mitosis. Protein S circulates free in plasma and, unlike Protein S in human and rat, does not complex with C4BP. Mouse Protein S precursor is 675 amino acids (aa) in length. It contains a signal sequence (aa 1-24), a cleavable prosegment (aa 25-41) and a 634 aa mature region. The mature molecule contains one Gla region (aa 42-87), four EGF-like domains (aa 117-283) and two laminin G-like regions (aa 299-665). Over aa 1-169 of the mature molecule, mouse Protein S shares 80% and 88% aa identity with human and rat Protein S, respectively.
Alternate Names
PROS1
Gene Symbol
PROS1
UniProt
Additional Protein S/PROS1 Products
Product Documents for Mouse Protein S/PROS1 Antibody
Product Specific Notices for Mouse Protein S/PROS1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...