bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

RAMP2 in Mouse Lung.

RAMP2 was detected in perfusion fixed frozen sections of mouse lung using Rat Anti-Mouse RAMP2 Monoclonal Antibody (Catalog # MAB6500) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse RAMP2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: bEnd.3 mouse endothelioma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse lung

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAMP2

RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.

Long Name

Receptor Activity Modifying Protein 2

Alternate Names

calcitonin receptor-like receptor activity modifying protein 2, Calcitonin-receptor-like receptor activity-modifying protein 2, CRLR activity-modifying protein 2, receptor (calcitonin) activity modifying protein 2, receptor (G protein-coupled) activity modifying protein 2, receptor activity modifying protein 2, receptor activity-modifying protein 2, receptor-activity-modifying protein 2

Entrez Gene IDs

10266 (Human); 54409 (Mouse); 58966 (Rat)

Gene Symbol

RAMP2

UniProt

Q9WUP0 (Mouse)

Additional RAMP2 Products

Product Documents for Mouse RAMP2 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse RAMP2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse RAMP2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6500

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all RAMP2 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse RAMP2 Antibody

Detection of RAMP2 in bEnd.3 Mouse Cell Line by Flow Cytometry.