Mouse/Rat alpha 1B-Glycoprotein Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8537
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse alpha 1B-Glycoprotein
Leu21-Gly512
Accession # Q19LI2
Leu21-Gly512
Accession # Q19LI2
Specificity
Detects mouse and rat alpha 1B-Glycoprotein in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human alpha 1B-Glycoprotein observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse/Rat alpha 1B-Glycoprotein Antibody
Detection of Mouse and Rat alpha 1B-Glycoprotein by Western Blot.
Western blot shows mouse plasma and rat plasma. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for a 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
alpha 1B-Glycoprotein in Hepa 1‑6 Mouse Cell Line.
a 1B-Glycoprotein was detected in immersion fixed Hepa 1-6 mouse hepatoma cell line using Rabbit Anti-Mouse/Rat a 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) at 1:20 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and rat alpha 1B-Glycoprotein by Simple WesternTM.
Simple Western lane view shows lysates mouse plasma and rat plasma, loaded at 0.2 mg/mL. A specific band was detected for alpha 1B-Glycoprotein at approximately 113 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse/Rat alpha 1B-Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8537) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse/Rat alpha 1B-Glycoprotein Antibody
Application
Recommended Usage
Immunocytochemistry
1:20-1:50 dilution
Sample: Immersion fixed Hepa 1‑6 mouse hepatoma cell line
Sample: Immersion fixed Hepa 1‑6 mouse hepatoma cell line
Simple Western
1:100 dilution
Sample: Mouse plasma and rat plasma
Sample: Mouse plasma and rat plasma
Western Blot
1:1000 dilution
Sample: Mouse plasma and rat plasma
Sample: Mouse plasma and rat plasma
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: alpha 1B-Glycoprotein
Alternate Names
A1B, A1BG, ABG, alpha 1BGlycoprotein, GAB
Gene Symbol
A1BG
UniProt
Additional alpha 1B-Glycoprotein Products
Product Documents for Mouse/Rat alpha 1B-Glycoprotein Antibody
Product Specific Notices for Mouse/Rat alpha 1B-Glycoprotein Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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