Mouse/Rat Carboxylesterase 3/CES3 Antibody

Name: Mouse/Rat Carboxylesterase 3/CES3 Antibody
Brand: R&D Systems
SKU: AF5985

R&D Systems, part of Bio-Techne | Catalog # AF5985

Submit a review

Citations (2)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Carboxylesterase 3/CES3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 3/CES3
Tyr19-Glu561
Accession # Q8VCT4

Specificity

Detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. Detects intrinsic CES3 in mouse and rat tissues in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat Carboxylesterase 3/CES3 Antibody

Detection of Mouse and Rat Carboxylesterase 3/CES3 by Western Blot.

Western blot shows lysates of mouse lung tissue and rat lung tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Carboxylesterase 3/CES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carboxylesterase 3/CES3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse/Rat Carboxylesterase 3/CES3 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carboxylesterase 3/CES3 (Catalog # 5428-CE), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Mouse lung tissue and rat lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxylesterase 3/CES3

Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue (3). Mouse CES3 shares 93% homology with rat CES3.

References

Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today. 10:313.
Satoh, T. and Hosokawa, M. (2006) Chem.-Biol. Interactions. 162:195.
Soni, K. G. et al. (2004) J. Biol. Chem. 279:40683.

Alternate Names

CES3

Entrez Gene IDs

23491 (Human); 104158 (Mouse); 113902 (Rat)

Gene Symbol