Mouse/Rat FABP2/I-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1486
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat FABP2/I‑FABP
Met1-Glu132
Accession # P02693
Met1-Glu132
Accession # P02693
Specificity
Detects mouse and rat FABP2/I‑FABP in Western blots. Detects rat FABP2/I‑FABP in direct ELISAs. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant rat FABP1, recombinant human FABP3, recombinant mouse (rm) FABP4, and rmFABP5 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat FABP2/I-FABP Antibody
Detection of Mouse FABP2/I‑FABP by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP2/I-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP2/I‑FABP in Rat Mesenchymal Stem Cells.
FABP2/I-FABP was detected in immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
FABP2/I‑FABP in Mouse Intestine.
FABP2/I-FABP was detected in immersion fixed frozen sections of adult mouse intestine using Goat Anti-Mouse/Rat FABP2/I-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1486) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to intestinal epithelia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse/Rat FABP2/I-FABP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes and Caco‑2 human colorectal adenocarcinoma cell line
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes and Caco‑2 human colorectal adenocarcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of adult mouse intestine
Sample: Immersion fixed frozen sections of adult mouse intestine
Simple Western
20 µg/mL
Sample: Mouse Small Intestine Issue
Sample: Mouse Small Intestine Issue
Western Blot
1 µg/mL
Sample: Mouse small intestine tissue
Sample: Mouse small intestine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP2/I-FABP
Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Alternate Names
I-FABP, IFABP, Intestinal FABP
Gene Symbol
FABP2
UniProt
Additional FABP2/I-FABP Products
Product Documents for Mouse/Rat FABP2/I-FABP Antibody
Product Specific Notices for Mouse/Rat FABP2/I-FABP Antibody
For research use only
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