Mouse/Rat GABAB R2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB104561

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB104561-100
MAB104561-SP
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2605F

View all GABA-B R2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482
Accession # O88871

Specificity

Detects rat GABAB R2 in direct ELISAs. Detects mouse and rat GABAB R2 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse/Rat GABAB R2 Antibody

Detection of Mouse and Rat GABAB R2 antibody by Western Blot.

Detection of Mouse and Rat GABAB R2 by Western Blot.

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for GABAB R2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of Rat GABAB R2 antibody by Simple WesternTM.

Detection of Rat GABAB R2 by Simple WesternTM.

Simple Western lane view shows lysates of rat brain tissue, loaded at 0,.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Mouse/Rat GABAB R2 Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Rat brain tissue

Western Blot

1 µg/mL
Sample: Mouse brain tissue and Rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from egg yolks

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Long Name

gamma-Aminobutyric Acid Type B Receptor Subunit 2

Alternate Names

GABABR2, GABBR2, GPR51, GPRC3B

Entrez Gene IDs

9568 (Human); 83633 (Rat)

Gene Symbol

GABBR2

UniProt

O88871

Additional GABA-B R2 Products

Product Documents for Mouse/Rat GABAB R2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat GABAB R2 Antibody

For research use only

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