Mouse/Rat Jak1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF602
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Jak1
Ala14-Tyr55 (predicted)
Accession # P52332
Ala14-Tyr55 (predicted)
Accession # P52332
Specificity
Detects mouse and rat Jak1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Jak1 Antibody
Detection of Mouse/Rat Jak1 by Western Blot.
Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line, Y3-Ag rat myeloid cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Jak1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF602) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Jak1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Jak1 in Mouse Splenocytes.
Jak1 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat Jak1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF602) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse/Rat Jak1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
1 µg/mL
Sample: Rat-2 rat embryonic fibroblast cell line, Y3-Ag rat myeloid cell line, and L6 rat myoblast cell line
Sample: Rat-2 rat embryonic fibroblast cell line, Y3-Ag rat myeloid cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jak1
Long Name
Janus Kinase 1
Alternate Names
EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3
Entrez Gene IDs
3716 (Human)
Gene Symbol
JAK1
UniProt
Additional Jak1 Products
Product Documents for Mouse/Rat Jak1 Antibody
Product Specific Notices for Mouse/Rat Jak1 Antibody
This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.
For research use only
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