Mouse/Rat Phospho-DARPP-32 (T34) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2847
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing mouse DARPP-32 T34 site
Specificity
Detects mouse and rat DARPP-32 when phosphorylated at T34 in Western blots. There is weak cross-reactivity with phosphorylated PP1 Inhibitor-1, which contains a nearly identical phosphorylation site. Reactivity with human Phospho-DARPP-32 has not been confirmed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse/Rat Phospho-DARPP-32 (T34) Antibody
Detection of Rat and Mouse Phospho-DARPP-32 (T34) by Western Blot.
Western blot shows lysates of rat and mouse corpus striata tissue untreated (-) or treated (+) with calcium for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse/Rat Phospho-DARPP-32 (T34) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2847), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DARPP-32 (T34) at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat Phospho-DARPP-32 (T34) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Calcium-treated rat and mouse corpus striata tissue
Sample: Calcium-treated rat and mouse corpus striata tissue
Reviewed Applications
Read 1 review rated 5 using AF2847 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DARPP-32
Dopamine- and cAMP-Regulated Neuronal Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein which anomalously migrates at about 32 - 35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), it is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons. Phosphorylation of DARPP-32 at T34 has been used as an index of PKA activation.
Long Name
Dopamine and cAMP-regulated Phosphoprotein
Alternate Names
DARPP32, PPP1R1B
Gene Symbol
PPP1R1B
Additional DARPP-32 Products
Product Documents for Mouse/Rat Phospho-DARPP-32 (T34) Antibody
Product Specific Notices for Mouse/Rat Phospho-DARPP-32 (T34) Antibody
For research use only
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