Mouse/Rat TNF RI/TNFRSF1A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB425
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Western Blot
Cited:
ELISA Development, ELISA Development (Capture), Luminex Development, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 47803
Product Specifications
Immunogen
E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118
Ile22-Ala212
Accession # P25118
Specificity
Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse/Rat TNF RI/TNFRSF1A Antibody
Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot.
Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse/Rat TNF RI/TNFRSF1A Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: C6 rat glioma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Mouse TNF RI/TNFRSF1A Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A
Gene Symbol
TNFRSF1A
UniProt
Additional TNF RI/TNFRSF1A Products
Product Documents for Mouse/Rat TNF RI/TNFRSF1A Antibody
Product Specific Notices for Mouse/Rat TNF RI/TNFRSF1A Antibody
For research use only
Loading...
Loading...
Loading...
Loading...