Jurkat human acute T cell leukemia cell line transfected with (A) mouse ROBO4 or (B) irrelevant transfectant and eGFP was stained with Rat Anti-Mouse ROBO4 Monoclonal Antibody (Catalog # MAB50041) followed by APC-conjugated Goat anti-Rat IgG secondary antibody Catalog # F0113. Quadrant markers were set based on Rat IgG2A Isotype Control (Catalog # MAB006, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ROBO4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

Roundabout receptors are molecular guidance proteins that function by interaction with Slit proteins to regulate axon guidance, neuronal migration, and leukocyte chemotaxis. ROBO4 expression is restricted to the endothelium and may also play a role in angiogenesis and endothelial cell migration.

Long Name

Roundabout Homolog 4

Alternate Names

ECSM4, FLJ20798, Magic roundabout, MGC133352, MGC133353, MRB, roundabout homolog 4, roundabout homolog 4, magic roundabout (Drosophila)

Entrez Gene IDs

54538 (Human); 74144 (Mouse)

Gene Symbol

ROBO4

UniProt

Q8C310

Additional ROBO4 Products

Product Documents for Mouse ROBO4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse ROBO4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse ROBO4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB50041

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ROBO4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse ROBO4 Antibody

Detection of ROBO4 in Jurkat Human Cell Line Transfected with Mouse ROBO4 and eGFP by Flow Cytometry.