Mouse S100A8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3059
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
ELISA Development, IHC-F, Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse S100A8
Pro2-Glu89
Accession # P27005
Pro2-Glu89
Accession # P27005
Specificity
Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse S100A8 Antibody
Detection of Mouse S100A8 by Western Blot.
Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A8 in Mouse Splenocytes.
S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
S100A8 in NMuMG Mouse Cell Line.
S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Mouse S100A8 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed NMuMG mouse mammary gland epithelial cell line and immersion fixed mouse splenocytes
Sample: Immersion fixed NMuMG mouse mammary gland epithelial cell line and immersion fixed mouse splenocytes
Simple Western
2.5 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Western Blot
0.25 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3.5 using AF3059 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A8
Long Name
S100 Calcium Binding Protein A8
Alternate Names
Calgranulin A, CFAG, MRP8
Gene Symbol
S100A8
UniProt
Additional S100A8 Products
Product Documents for Mouse S100A8 Antibody
Product Specific Notices for Mouse S100A8 Antibody
For research use only
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