Mouse S100A9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2065
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
ELISA Development, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 372510
Product Specifications
Immunogen
E. coli-derived recombinant mouse S100A9
Met1-Lys113
Accession # P31725
Met1-Lys113
Accession # P31725
Specificity
Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A10, rhS100B, or rhS100P is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse S100A9 Antibody
Detection of Mouse S100A9 by Western Blot.
Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse S100A9 by Simple Western™
Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse S100A9 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Mouse spleen tissue
Sample: Mouse spleen tissue
Western Blot
2 µg/mL
Sample: Mouse lung tissue
Sample: Mouse lung tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A9
S100A9, also known as calgranulin B or migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
UniProt
Additional S100A9 Products
Product Documents for Mouse S100A9 Antibody
Product Specific Notices for Mouse S100A9 Antibody
For research use only
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