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Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7089N

R&D Systems, part of Bio-Techne
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FAB7089N

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 713412

Product Specifications

Immunogen

Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530

Specificity

Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images

Detection of S1P1/EDG-1 antibody in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and GFP antibody by Flow Cytometry.

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and GFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2AAlexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P1/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB7089N) or (B) Rat IgG2AAlexa Fluor 700 Isotype Control (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP, and mouse thymocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using FAB7089N in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

Additional S1P1/EDG-1 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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