Mouse S1P1/EDG-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7089
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 713412
Product Specifications
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Accession # O08530
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse S1P1/EDG-1 Antibody
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry.
Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.S1P1/EDG‑1 in Mouse Heart.
S1P1/EDG-1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse S1P1/EDG-1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP, and mouse thymocytes
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP, and mouse thymocytes
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse heart
Sample: Perfusion fixed frozen sections of mouse heart
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S1P1/EDG-1
Long Name
Endothelial Differentiation Gene 1
Alternate Names
CD363, EDG1, S1PR1
Gene Symbol
S1PR1
UniProt
Additional S1P1/EDG-1 Products
Product Documents for Mouse S1P1/EDG-1 Antibody
Product Specific Notices for Mouse S1P1/EDG-1 Antibody
For research use only
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