Mouse SDNSF/MCFD2 Antibody

Name: Mouse SDNSF/MCFD2 Antibody
Brand: R&D Systems
SKU: AF2556

R&D Systems, part of Bio-Techne | Catalog # AF2556

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Biotin

BAF2556

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SDNSF/MCFD2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
His27-Gln145
Accession # Q8K5B2

Specificity

Detects mouse SDNSF/MCFD2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse SDNSF/MCFD2 Antibody

SDNSF/MCFD2 in Embryonic Mouse Brain.

SDNSF/MCFD2 was detected in immersion fixed frozen sections of embryonic mouse brain (E10, neural tube) using Goat Anti-Mouse SDNSF/MCFD2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2556) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to secretion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse SDNSF/MCFD2 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse brain (E10, neural tube)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SDNSF/MCFD2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), was described as a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 (Mannose-binding lectin-1, also known as ERGIC-53) complex that functions as a specific cargo receptor for the ER to golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency. Human SDNSF shares 84% and 82% amino acid sequence identity with rat and mouse SDNSF, respectively.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Alternate Names

MCFD2

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Gene Symbol