Mouse Semaphorin 3B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5440
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 904201
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3B
Pro26-Pro722
Accession # Q62177
Pro26-Pro722
Accession # Q62177
Specificity
Detects mouse Semaphorin 3B in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Semaphorin 3B Antibody
Detection of Mouse Semaphorin 3B by Western Blot.
Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Semaphorin 3B in NS0 Mouse Cell Line.
Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse Semaphorin 3B Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed NSO mouse myeloma cell line transfected with mouse Semaphorin 3B or irrelevant transfectant
Sample: Immersion fixed NSO mouse myeloma cell line transfected with mouse Semaphorin 3B or irrelevant transfectant
Western Blot
0.5 µg/mL
Sample: NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B
Sample: NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB5440 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 3B
Alternate Names
LUCA-1, SEMA3B, SEMA5, SEMAA, SEMAV
Gene Symbol
SEMA3B
UniProt
Additional Semaphorin 3B Products
Product Documents for Mouse Semaphorin 3B Antibody
Product Specific Notices for Mouse Semaphorin 3B Antibody
For research use only
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