Mouse cortical stem cells were stained with Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Detection of Semaphorin 4F in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Semaphorin 4F in Mouse Spinal Cord.

Semaphorin 4F was detected in perfusion fixed frozen sections of mouse spinal cord using Rat Anti-Mouse Semaphorin 4F Monoclonal Antibody (Catalog # MAB7200) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to synaptic puncta. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Semaphorin 4F Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µL/10⁶ cells
Sample: Mouse cortical stem cells and bEnd.3 mouse endothelioma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4F

Semaphorin 4F (Sema4F; also Sema W) is a 90-100 kDa, class IV member of the semaphorin family of proteins. It is expressed by select cell types, including Schwann cells and neurons, and likely acts as an inhibitor of cell proliferation by Schwann cells, and a regulator of postsynaptic glutamatergic synapses on neuron dendrites. Mature mouse Sema4F is a type I transmembrane protein that is 737 amino acids (aa) in length. It contains a 627 aa extracellular region (aa 41-667) that is characterized by the presence of one Sema domain (aa 71-493), a PSI region (aa 518-569), and a C2-type Ig-like domain (aa 586-641). Over aa 41-667, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively.

Alternate Names

PRO2353, SEMA4F, SEMAM, SEMAW

Entrez Gene IDs

10505 (Human); 20355 (Mouse); 29745 (Rat)

Gene Symbol

SEMA4F

UniProt

Q9Z123 (Mouse)

Additional Semaphorin 4F Products

Product Documents for Mouse Semaphorin 4F Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse Semaphorin 4F Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse Semaphorin 4F Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7200

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse Semaphorin 4F Antibody

Detection of Semaphorin 4F in Mouse Cortical Stem Cells by Flow Cytometry.