Mouse Serpin A1a Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7690
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Transgenic Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 882213
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A1a
Met1-Lys413
Accession # P07758
Met1-Lys413
Accession # P07758
Specificity
Detects mouse Serpin A1a in ELISAs.
In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Serpin A1c, approximately 15% cross-reactivity with recombinant human A1, and less than 5% cross-reactivity with rmSerpin A3n is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse Serpin A1a Antibody
Detection of Mouse SerpinA1a by Western Blot.
Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Serpin A1a at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin A1a by Simple WesternTM.
Simple Western lane view shows mouse serum, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1a at approximately 62 kDa (as indicated) using 2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse Serpin A1a Antibody
Application
Recommended Usage
Simple Western
2 µg/mL
Sample: Mouse serum
Sample: Mouse serum
Western Blot
0.2 µg/mL
Sample: Mouse serum
Sample: Mouse serum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin A1a
References
- Gettins, P.G. (2002) Chem. Rev. 102:4751.
- Kalsheker N.A. (1989) Biosci. Rep. 9:129. 3. Ritchie, R.F. et al. (2000) J. Clin. Lab. Anal. 14:284.
Alternate Names
Aat2, Dom1, PI1, Spi1-1
Gene Symbol
SERPINA1
UniProt
Additional Serpin A1a Products
Product Documents for Mouse Serpin A1a Antibody
Product Specific Notices for Mouse Serpin A1a Antibody
For research use only
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