Mouse Sonic Hedgehog/Shh C-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB445
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 55626
Product Specifications
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog
Cys199-Ser437
Accession # Q62226.1
Cys199-Ser437
Accession # Q62226.1
Specificity
Detects recombinant mouse Shh C-terminal peptide in direct ELISAs and Western blots. Does not cross-react in direct ELISAs with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide, or rmShh N-terminal peptide.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Sonic Hedgehog/Shh C-Terminus Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse Sonic Hedgehog C-Terminus
Sample: Recombinant Mouse Sonic Hedgehog C-Terminus
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. It is cleaved by autoproteolysis to release an N-terminal signaling peptide that binds the multicomponent receptor complex containing the multi-pass transmembrane proteins Patched and Smoothened. The C-terminal peptide carries the protease domain which acts only in CIS. It also acts on a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N.
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for Mouse Sonic Hedgehog/Shh C-Terminus Antibody
Product Specific Notices for Mouse Sonic Hedgehog/Shh C-Terminus Antibody
For research use only
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