Mouse TEX19.1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB66451

R&D Systems, part of Bio-Techne
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MAB66451
MAB66451-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 678826

View all TEX19.1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TEX19.1
Arg189-Pro351
Accession # Q99MV2

Specificity

Detects mouse TEX19.1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse TEX19.1 Antibody

Detection of Mouse TEX19.1 antibody by Western Blot.

Detection of Mouse TEX19.1 by Western Blot.

Western blot shows lysates of mouse testis tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TEX19.1 Monoclonal Antibody (Catalog # MAB66451) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TEX19.1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TEX19 by Immunocytochemistry (ICC).1 antibody in D3 Mouse Embryonic Stem Cells by Immunocytochemistry (ICC).

TEX19.1 in D3 Mouse Embryonic Stem Cells.

TEX19.1 was detected in immersion fixed D3 mouse embryonic stem cells using Rat Anti-Mouse TEX19.1 Monoclonal Antibody (Catalog # MAB66451) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse TEX19.1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cells

Western Blot

1 µg/mL
Sample: Mouse testis tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEX19.1

Testis-expressed protein 19.1 (TEX19.1; also Tex19a and Tex19) is a 40 kDa (calculated) pluripotent stem cell molecule. Mouse TEX19.1 is 351 amino acids (aa) in length.  The first conserved domain is localized on the N-terminal boundary of the protein and is 58 residues long.  It is known as the MCP domain, and begins with an invariant MCPPVS motif. The second conserved domain, known as the VPTEL domain, begins with an invariant VPTEL motif and is 38 amino acids long. Mouse TEX19.1 shares 70% and 24% aa identity with rat and human Tex19.1, respectively. The protein is restricted to mammals and is expressed in testis, placenta, and ovary.  It is expressed in early embryo and is later limited to the germ line. TEX19.1 has been shown to present in the cytoplasm of spermatogonia and early spermatocytes in adult mouse testes (1). TEX19.1 is thought to be a transcription factor regulating target genes responsible for pluripotency; alternatively, it may have a role in controlling the chromatin architecture of the pluripotent nucleus.

References

  1. Ollinger R. et. al. (2008) PLos 4(9):e1000199.

Long Name

Testis Expressed 19.1

Alternate Names

Tex19a

Entrez Gene IDs

73679 (Mouse)

Gene Symbol

TEX19.1

UniProt

Q99MV2

Additional TEX19.1 Products

Product Documents for Mouse TEX19.1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse TEX19.1 Antibody

For research use only

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