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Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 786404

Product Specifications

Immunogen

E. coli-derived recombinant mouse TLR11
Thr209-Gln325
Accession # Q6R5P0

Specificity

Detects mouse TLR11 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse TLR11 Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25-1 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TLR11

TLR11 is a type I transmembrane receptor of the Toll-like receptor family that is primarily expressed in epithelial cells, but also in dendritic cells and macrophages. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats and 9 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 184 aa cytoplasmic domain with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER), interacts with the multispan ER protein UNC93B1, recognizes profilin-like proteins on Toxoplasma gondii and other intracellular parasites, and activates dendritic cell IL-12 production.

Long Name

Toll-like Receptor 11

Alternate Names

Gm287

Entrez Gene IDs

239081 (Mouse); 498491 (Rat)

Gene Symbol

TLR11

UniProt

Additional TLR11 Products

Product Documents for Mouse TLR11 Alexa Fluor® 488-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TLR11 Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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