RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 Monoclonal Antibody (Catalog # MAB7640, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse TLR11 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR11

TLR11 is a type I transmembrane receptor of the Toll-like receptor family that is primarily expressed in epithelial cells, but also in dendritic cells and macrophages. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats and 9 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 184 aa cytoplasmic domain with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER), interacts with the multispan ER protein UNC93B1, recognizes profilin-like proteins on Toxoplasma gondii and other intracellular parasites, and activates dendritic cell IL-12 production.

Long Name

Toll-like Receptor 11

Alternate Names

Gm287

Entrez Gene IDs

239081 (Mouse); 498491 (Rat)

Gene Symbol

TLR11

UniProt

Q6R5P0

Additional TLR11 Products

Product Documents for Mouse TLR11 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TLR11 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TLR11 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7640

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TLR11 Antibody

Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry.