TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel; positive staining) and untreated (right panel; negative staining) using Rat Anti-Mouse TREM-1 Biotinylated Monoclonal Antibody (Catalog # BAM1187) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse TREM-1 Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes stimulated with LPS

Western Blot

1 µg/mL
Sample: Recombinant Mouse TREM-1 Fc Chimera (Catalog # 1187-TR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References

Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9JKE2

Additional TREM-1 Products

Product Documents for Mouse TREM-1 Biotinylated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TREM-1 Biotinylated Antibody

For research use only

Citations
Reviews

Mouse TREM-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM1187

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Mouse TREM-1 Biotinylated Antibody

TREM‑1 in Mouse Splenocytes.