Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse TREM-1 PE-conjugated Antibody

Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1187P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TREM-1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References

Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Alternate Names

CD354, TREM1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Gene Symbol

TREM1

UniProt

Q9JKE2

Additional TREM-1 Products

Product Documents for Mouse TREM-1 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse TREM-1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Mouse TREM-1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1187P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications