Detects mouse TREML2/TLT-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) TREM-1, -2, -2b, -3, -4, rmTREML1/TLT-1, rmTREML4/TLT-4, or recombinant human (rh) NKp44 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse TREML2/TLT-2 Antibody

Detection of TREML2 in M1 Mouse Cell Line by Flow Cytometry.

M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse TREML2/TLT-2 Monoclonal Antibody (Catalog # MAB6707, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Applications for Mouse TREML2/TLT-2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: M1 mouse myeloid leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML2/TLT-2

TREML2, also called TLT-2 (Trem-like transcript 2) is a 33 kDa (predicted) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8⁺ T cells.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 2

Alternate Names

TLT2

Entrez Gene IDs

79865 (Human); 328833 (Mouse)

Gene Symbol

TREML2

UniProt

Q2LA85

Additional TREML2/TLT-2 Products

Product Documents for Mouse TREML2/TLT-2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TREML2/TLT-2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TREML2/TLT-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6707

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TREML2/TLT-2 Antibodies »

Product Specifications

Immunogen

Specificity