Mouse USP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5804
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse USP2 isoform 2
Lys47-Met396
Accession # NP_932759
Lys47-Met396
Accession # NP_932759
Specificity
Detects mouse USP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human USP2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse USP2 Antibody
USP2 in NIH‑3T3 Mouse Cell Line.
USP2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse USP2 by Western Blot.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for USP2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
USP2 in Mouse Kidney.
USP2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse USP2 Antibody
Application
Recommended Usage
Immunocytochemistry
0.3-25 µg/mL
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Sample: Immersion fixed NIH-3T3 mouse embryonic fibroblast cell line
Immunohistochemistry
3-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney
Sample: Perfusion fixed frozen sections of mouse kidney
Western Blot
1 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: USP2
Long Name
Ubiquitin-specific Protease 2
Alternate Names
UBP41, USP9
Gene Symbol
USP2
UniProt
Additional USP2 Products
Product Documents for Mouse USP2 Antibody
Product Specific Notices for Mouse USP2 Antibody
For research use only
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