Mouse VCAM-1/CD106 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF643
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Cell Selection, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VCAM‑1/CD106
Phe25-Glu698
Accession # P29533
Phe25-Glu698
Accession # P29533
Specificity
Detects mouse VCAM‑1/CD106 in direct ELISAs and Western blots. In direct ELISA and Western blots, approximately 5% cross-reactivity with recombinant human VCAM-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse VCAM-1/CD106 Antibody
Detection of Mouse VCAM‑1/CD106 by Western Blot.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for VCAM-1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VCAM‑1/CD106 in Mouse Embryo.
VCAM-1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of VCAM‑1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry.
Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).
Applications for Mouse VCAM-1/CD106 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow cells and bEND.3 cells
Sample: Mouse bone marrow cells and bEND.3 cells
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cerebellum) and immersion fixed frozen sections of mouse embryo
Sample: Perfusion fixed frozen sections of rat brain (cerebellum) and immersion fixed frozen sections of mouse embryo
Simple Western
1 µg/mL
Sample: C2C12 mouse myoblast cell line and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Sample: C2C12 mouse myoblast cell line and 3T3‑L1 mouse embryonic fibroblast adipose-like cell line
Western Blot
0.25 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 3.6 using AF643 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4 beta7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
UniProt
Additional VCAM-1/CD106 Products
Product Documents for Mouse VCAM-1/CD106 Antibody
Product Specific Notices for Mouse VCAM-1/CD106 Antibody
For research use only
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