MRP1 Antibody (IU2H10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB400-156

Novus Biologicals, part of Bio-Techne
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NB400-156-0.1ml
NB400-156SS
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

CyTOF-ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Cited:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # IU2H10

Format

BSA Free

Concentration

1.0 mg/ml

View all MRP1 Antibodies »

Product Specifications

Immunogen

Recombinant peptide (a.a. 1-33) of human MRP1. [UniProt# P33527].

Epitope

Amino acids 8-17 of human MRP1.

Localization

Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MRP1 Antibody (IU2H10) - BSA Free

Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.
Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) - BSA Free [NB400-156]

Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) - BSA Free [NB400-156]

Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Western Blot: MRP1 Antibody (IU2H10)BSA Free [NB400-156]

Western Blot: MRP1 Antibody (IU2H10)BSA Free [NB400-156]

Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)

Applications for MRP1 Antibody (IU2H10) - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature

Flow Cytometry

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunoprecipitation

reported in scientific literature

Western Blot

1:500
Application Notes
In Western blot a band was observed at ~170-190 kDa in transfected samples. There may be a lower MW band that is a degradation product. This antibody is CyTOF ready. See Simple Western Antibody Database for Simple Western validation: Tested in brain; separated by size; antibody dilution of 1:100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MRP1

The MRP molecule, an integral membrane glycophosphoprotein belonging to the same superfamily of ATP-binding cassette transmembrane transporter proteins as P-glycoprotein, is overexpressed in many P-glycoprotein-negative, multidrug resistant cell lines and tumours. It is believed that the main function of MRP in drug resistance is that of a plasma membrane drug efflux pump.

Long Name

Multi-drug Resistance Protein 1

Alternate Names

ABCC1, GS-X

Gene Symbol

ABCC1

Additional MRP1 Products

Product Documents for MRP1 Antibody (IU2H10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MRP1 Antibody (IU2H10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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