MRP1 Antibody (IU2H10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80857
![Western Blot: MRP1 Antibody (IU2H10)Azide and BSA Free [NBP2-80857]](https://resources.bio-techne.com/images/products/MRP1-Antibody-IU2H10-Azide-and-BSA-Free-Western-Blot-NBP2-80857-img0001.jpg "Western Blot: MRP1 Antibody (IU2H10)Azide and BSA Free [NBP2-80857]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
CyTOF-ready, Dual RNAscope ISH-IHC, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # IU2H10
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for MRP1 Antibody (IU2H10) - Azide and BSA Free
Immunogen
Recombinant peptide (a.a. 1-33) of human MRP1. [UniProt# P33527].
Epitope
Amino acids 8-17 of human MRP1.
Localization
Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MRP1 Antibody (IU2H10) - Azide and BSA Free
Western Blot: MRP1 Antibody (IU2H10)Azide and BSA Free [NBP2-80857]
Western Blot: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.) Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857]](https://resources.bio-techne.com/images/products/MRP1-Antibody-IU2H10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80857-img0002.jpg "Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857]")
Immunocytochemistry/ Immunofluorescence: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857]
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively. Image from the standard format of this antibody.
Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) - Azide and BSA Free [NBP2-80857] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells. Image from the standard format of this antibody.
Applications for MRP1 Antibody (IU2H10) - Azide and BSA Free
Application
Recommended Usage
ELISA
reported in scientific literature
Flow Cytometry
reported in scientific literature
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunoprecipitation
reported in scientific literature
Western Blot
1:500
Application Notes
In Western blot a band was observed at ~170-190 kDa in transfected samples. There may be a lower MW band that is a degradation product. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MRP1
Long Name
Multi-drug Resistance Protein 1
Alternate Names
ABCC1, GS-X
Gene Symbol
ABCC1
Additional MRP1 Products
Product Documents for MRP1 Antibody (IU2H10) - Azide and BSA Free
Product Specific Notices for MRP1 Antibody (IU2H10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...